Pine-pollen-MPN

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6-step dilution tests measure the concentration of thraustochytrids in the seawater samples with an estimate called the most probable number (MPN).  The thraustochytrids were picked up by the pine-pollen baiting method from each diluted solution.  After 1-week incubation, the pine-pollens were spread on the agar plates.  In the case of the presence of thraustochytrids, the colonies were appeared within a week.